Exploring the Potential of α-Amylase: Genetic Identification, Purification Techniques and Its Applications

α Amylase is a key enzyme widely employed in various industrial applications, including foodstuffs, fabric goods, and biofuel production. In our review study, we focused on genetically characterized microbial isolates that produce
-amylase from diverse sources. DNA isolation was performed using an alkaline lysis protocol followed by PCR amplification of the 16S rRNA gene. After isolating DNA samples, the 16S rRNA gene was PCR-amplified, and the phylogenetic relationships were determined through sequencing and subsequent analysis. To optimize enzyme production, various parameters such as pH, temperature, and incubation time were investigated. To purify the
-amylase enzyme, several purification techniques were employed. The crude amylase enzyme preparation was precipitated using ammonium sulfate. After dialysis and size exclusion chromatography, the purified enzyme preparation was analyzed further. To assess purity, the dialyzed samples were analyzed using a suitable chromatographic method to separate and evaluate the sample components.
-amylase characterization involved molecular weight determination by using SDS-PAGE, size determination by Size exclusion chromatography, and comprehensive examination was done by using mass spectrometry. In conclusion, this study successfully compared the genetically identified and characterized
-amylase producing microbial isolates. The optimized incubation conditions and purification techniques provided valuable insights into the production and purification of this industrially important enzyme.
-Amylase enzymes play an essential role in several industrial processes, improving and facilitating eco-friendly methods in areas such as textile, food, pharmaceutical, and alcohol manufacturing. Their adaptability drives cross-sector, sustainable innovations.Further studies can focus on enhancing the enzyme's characteristics and exploring its potential applications in various industries.